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Figure 1. Real time
PCR (TaqMan) validation of increased expression in 4N TP53MUT BE cells.
A series of genes (NEK2, p55CDC20, CENPA and RRM1) that were upregulated
in the 4N TP53MUT
cluster were assayed in triplicate in each of the replicate CP-B and CP-C
cultures.
Primers and probes were designed using ???? software. All values were normalized
using GAPDH.
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