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Cancer Research Supplemental Data
Molecular phenotype of spontaneously-arising G2/tetraploid intermediates
of neoplastic progression in Barrett's oesophagus.
Michael T. Barrett, David Pritchard, Corinna Palanca-Wessels,
Judy Anderson, Brian J. Reid, and Peter S. Rabinovitch
Cancer Research 2003 63: p. 4211-17 
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Image Files
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Figure 1. Real
time PCR (TaqMan) validation of increased expression in 4N TP53MUT
BE cells. A series of genes (NEK2, p55CDC20, CENPA and RRM1)
that were upregulated in the 4N TP53MUT cluster were assayed
in triplicate in each of the replicate CP-B and CP-C cultures.
Primers and probes were designed using Primer Express™ software.
All values were normalized using GAPDH.
click image for full version
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Figure
2A. Hierarchical clustering of the absolute
expression values for all genes and all 20 hybridizations in the dataset.
Region “A” indicates a cluster of 42 genes that was up regulated
in both 2N and 4N TP53wt cells compared to the TP53mut cell populations.
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Figure
2B. Expanded view of the cluster of 42
genes that was up regulated in both 2N and 4N TP53wt cells compared
to the TP53mut cell populations.
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image for full version
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Figure
3. Hierarchical clustering of the 135
genes over expressed (top main branch of the tree) and 452 genes
under expressed (bottom main branch of the tree) in one or more
G2 cell culture.
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image for full version
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Data Files
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